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Related Experiment Videos

Menstruum induces changes in mesothelial cell morphology.

C A Koks1, A Y Demir Weusten, P G Groothuis

  • 1Department of Obstetrics and Gynecology, Academisch Ziekenhuis Maastricht, The Netherlands. jacques_carolien@yahoo.com

Gynecologic and Obstetric Investigation
|July 15, 2000
PubMed
Summary

Menstrual tissue remains viable in peritoneal fluid and damages the peritoneal lining. This damage to mesothelial cells creates sites for endometrial adhesion, suggesting menstruum is harmful.

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Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Gynecologic Pathology

Background:

  • Previous studies indicate preferential adhesion of menstrual endometrium to the peritoneum.
  • The mechanism causing peritoneal damage, whether pre-existing or inflicted by menstrual tissue, requires elucidation.

Purpose of the Study:

  • To investigate the hypothesis that menstrual tissue itself damages the peritoneum.
  • To evaluate the viability of menstrual endometrial tissue in peritoneal fluid (PF).
  • To study morphologic changes in mesothelial cells during in vitro cocultures with menstruum.

Main Methods:

  • Menstrual tissue viability assessed using MTT assay after culture in PF or medium.
  • Coculture of human omental mesothelial cells with menstrual endometrial fragments or serum.

Related Experiment Videos

  • Scanning electron microscopy used to evaluate mesothelial cell morphology.

    • Main Results:

    • Menstrual endometrial fragments maintained viability in PF for at least 72 hours.
    • Menstrual tissue and serum induced severe morphologic alterations in mesothelial cells, including retraction and gap formation.
    • Conditioned medium from menstrual endometrium also caused mesothelial cell changes.

    Conclusions:

    • Menstrual endometrial fragments are viable in peritoneal fluid.
    • Antegradely shed menstruum is harmful to the peritoneal lining, causing mesothelial cell damage.
    • This damage facilitates adhesion of menstrual endometrium to the peritoneum.