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Linkage mapping and comparative analysis of bovine expressed sequence tags (ESTs)
W M Grosse1, S M Kappes, R A McGraw
1Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
Animal Genetics
|July 15, 2000
Summary
Bovine expressed sequence tags (ESTs) with microsatellites serve as valuable genetic markers. This study successfully mapped these bovine ESTs, enhancing the density of gene markers on bovine linkage and comparative maps.
Area of Science:
- Genomics
- Molecular Biology
- Animal Genetics
Background:
- Bovine expressed sequence tags (ESTs) containing microsatellites are crucial for genetic mapping.
- Developing high-density genetic maps is essential for cattle breeding and research.
Purpose of the Study:
- To isolate and characterize bovine ESTs with microsatellites.
- To assess their utility for constructing bovine linkage and comparative maps.
- To enhance the density of gene markers on bovine maps.
Main Methods:
- Isolation of bovine EST clones from a skeletal muscle cDNA library using a (CA)10 probe.
- Sequencing of ESTs and BLAST analysis for homology to known mammalian sequences.
- Development of microsatellite markers and placement on the MARC bovine linkage map.
- Comparative mapping using human EST orthologs and cytogenetic data.
Main Results:
- 31 out of 47 bovine ESTs showed homology to known mammalian coding regions, with 30 matching human sequences.
- Microsatellites were found in 27 ESTs, leading to 11 developed markers on the bovine linkage map.
- Comparative mapping allowed inference of bovine map positions for 17 sequences.
Conclusions:
- Mapping bovine ESTs with microsatellites is a viable strategy to increase gene marker density.
- This approach aids in the development of more comprehensive bovine linkage and comparative maps.
- The identified ESTs contribute to a better understanding of bovine genome organization and function.