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In vitro testing of ten bone cements after different time intervals from polymerization
G Ciapetti1, D Granchi, S Stea
1Laboratorio di Tecnologia Medica, Istituti Ortopedici Rizzoli, Bologna, Italy. gabriela.ciapetti@ior.it
Journal of Biomaterials Science. Polymer Edition
|July 15, 2000
Summary
Two acrylic bone cements showed toxicity to L929 cells after 1-hour curing. Barium sulphate and high benzoyl peroxide concentrations were identified as potential cytotoxic agents in bone cement extracts.
Area of Science:
- Biomaterials Science
- Cell Biology
- Orthopedic Surgery
Background:
- The biological response of cells to implanted bone cement is critical for cemented implant success.
- Understanding bone cement cytotoxicity is essential for patient safety and implant longevity.
Purpose of the Study:
- To evaluate the in vitro cytotoxicity of ten orthopedic acrylic bone cements.
- To identify potential cytotoxic components within bone cements.
Main Methods:
- Assessed cytotoxicity of bone cement extracts using L929 cells.
- Employed neutral red uptake (NRU) and propidium iodide (PI) assays for cell viability.
- Analyzed extracts from cements cured for 1 hour and 7 days.
Main Results:
- CERIM LT and CMW2 cements were toxic after 1-hour curing; Zimmer-low viscosity cement also showed toxicity after 7-day curing.
- Barium sulphate and benzoyl peroxide (≥1%) were identified as cytotoxic components at the 1-hour endpoint.
- Toxic effects diminished with extract dilution, except for CERIM LT.
Conclusions:
- Specific bone cements exhibit cytotoxicity, influenced by curing time and components.
- Barium sulphate and high benzoyl peroxide concentrations are key contributors to early-stage bone cement cytotoxicity.
- Further investigation into bone cement formulation is warranted to minimize adverse cellular responses.