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Updated: Jul 10, 2026

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
Biogenic amines occurrence in wine. Amino acid decarboxylase and proteolytic activities expression by Oenococcus oeni
M C Leitão1, H C Teixeira, M T Barreto Crespo
1Instituto de Biologia Experimental e Tecnológica (IBET) and Instituto de Tecnologia Química e Biológica (ITQB)-Universidade Nova de Lisboa, Apt. 12, 2781-901 Oeiras, Portugal.
Abstract:
This work deals with the study of the proteolytic and amino acid decarboxylase activities of selected Oenococcus oeni isolates and the effect of yeast autolysis on biogenic amines production in wine. A total of 220 isolates of O. oeni were tested for decarboxylase and proteolytic activity. Only six isolates showed both activities, but only after a period of adaptation in a growth medium containing wine. The results reported on this paper show that proteolytic activity was dependent on medium composition and bacterial growth phase. It can be assumed that the ability of O. oeni to use wine peptides and to produce biogenic amines is not a constant characteristic of this species, and enzymatic system expression appears to be closely dependent on nutritional and energetical composition of the medium. It also seems to be strain dependent and not widespread among this bacterial community.
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