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The trouble with tribbles

L A Johnston1

  • 1B2-152, Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA. lajohnst@fred.fhcrc.org

Current Biology : CB
|July 19, 2000
PubMed

Insights

A new cell division inhibitor, Tribbles, stops mitosis during fruit fly development. Tribbles promotes the breakdown of String/Cdc25, a key cell cycle regulator.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Genetics

Background:

  • Cell division and cell movement are crucial for embryonic development.
  • Proper regulation of mitosis is essential for tissue formation and organismal integrity.

Purpose of the Study:

  • To identify novel regulators of cell division during development.
  • To elucidate the mechanism by which Tribbles affects mitosis in Drosophila.

Main Methods:

  • Genetic screening in Drosophila melanogaster to identify cell division inhibitors.
  • Mitotic assays to assess the effect of Tribbles on cell cycle progression.
  • Biochemical experiments to determine the molecular targets of Tribbles.

Main Results:

  • Identification of Tribbles as a novel inhibitor of cell division.
  • Tribbles blocks mitosis at a specific stage during Drosophila morphogenesis.
  • Data suggest Tribbles promotes the proteolytic degradation of String/Cdc25, a key mitotic regulator.

Conclusions:

  • Tribbles plays a critical role in coordinating cell division with morphogenesis.
  • The mechanism involves the regulation of String/Cdc25 stability.
  • Tribbles represents a potential target for understanding developmental cell cycle control.

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