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Cloning and recombinant expression of the La RNA-binding protein from Trypanosoma brucei
1Max Planck Institute for Biophysical Chemistry, Department of Biochemistry, Am Fassberg 11, 37077, Göttingen, Germany.
Abstract:
We report the isolation, cloning and recombinant expression of a Trypanosoma brucei homolog of the La RNA-binding protein. Based on peptide sequence information we have isolated a cDNA clone which encodes a protein of 335 amino acids with a predicted molecular weight of 37.7 kDa. The amino acid sequence fits the domain structure of known La proteins and contains a putative ATP-binding site located in the COOH-terminal domain. The cDNA was expressed as a glutathione S-transferase fusion protein in Escherichia coli, and the recombinant protein displayed RNA-binding activity in an electrophoretic mobility shift assay.