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Sequence and molecular analysis of the rpoA cluster genes from Xanthomonas campestris pv. campestris
J Y Lai1, C F Huang, Y H Tseng
1Institute of Molecular Biology, National Chung Hsing University, 402, Taichung, Taiwan.
Biochimica Et Biophysica Acta
|July 19, 2000
Abstract:
The Xanthomonas campestris rpsM (S13)-rpsK (S11)-rpsD (S4)-rpoA (alpha)-rplQ (L17) cluster, encoding RNA polymerase alpha-subunit and four ribosomal proteins, reside in a 3164-bp DNA region. The N-terminal sequence of the authentic alpha-protein determined chemically matches that predicted from the nucleotide sequence. rplQ is monocistronic, instead of being co-transcribed with the other genes as in Escherichia coli. Antiserum against the His-tagged alpha-protein cross-reacted with the E. coli alpha-protein.