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Related Experiment Videos

Antigenic and sequence diversity in gonococcal transferrin-binding protein A.

C N Cornelissen1, J E Anderson, I C Boulton

  • 1Department of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23298, USA. cncornel@hsc.vcu.edu

Infection and Immunity
|July 19, 2000
PubMed
Summary

Neisseria gonorrhoeae uses iron-binding proteins like transferrin for growth. This study analyzed the diversity of TbpA, a key protein in iron uptake, revealing antigenic variations among gonococcal strains.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Immunology

Background:

  • Neisseria gonorrhoeae requires iron and utilizes human iron-binding proteins like transferrin.
  • Iron uptake involves cell surface receptors, including transferrin-binding proteins TbpA and TbpB.
  • Understanding TbpA diversity is crucial for vaccine development and understanding gonococcal pathogenesis.

Purpose of the Study:

  • To assess sequence diversity of the tbpA gene among different Neisseria gonorrhoeae strains.
  • To identify conserved regions within TbpA important for receptor structure and function.
  • To evaluate the antigenic diversity of gonococcal strains using antipeptide sera targeting TbpA.

Main Methods:

  • Sequencing of four additional tbpA genes from N. gonorrhoeae strains.

Related Experiment Videos

  • Comparative sequence analysis of tbpA genes from various pathogenic bacteria.
  • Generation of polyclonal mouse sera against synthetic peptides of TbpA.
  • Western blot analysis to detect TbpA reactivity with sera.
  • Evaluation of antigenic diversity across gonococcal strains using antipeptide sera.

    • Main Results:

    • Sequence diversity was observed among the analyzed tbpA genes.
    • Conserved regions potentially important for TbpA structure and function were identified.
    • One antipeptide serum specifically recognized native TbpA on intact gonococci, indicating surface exposure.
    • Antigenic diversity among gonococcal strains was confirmed using the generated antipeptide sera.

    Conclusions:

    • The TbpA protein exhibits sequence and antigenic diversity among Neisseria gonorrhoeae strains.
    • Specific surface-exposed regions of TbpA can be targeted by antibodies.
    • Findings contribute to understanding gonococcal iron acquisition mechanisms and potential vaccine targets.