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Cellulase allergy and challenge tests with cellulase using immunologic assessment.
M Vanhanen1, T Tuomi, O Tupasela
1Finnish Institute of Occupational Health, Helsinki. markku.vanhanen@occuphealth.fi
Scandinavian Journal of Work, Environment & Health
|July 20, 2000
Summary
A new challenge test effectively diagnoses allergic asthma and rhinitis caused by cellulase exposure. This method simulates workplace conditions, accurately reproducing respiratory symptoms in sensitized individuals.
Area of Science:
- Occupational Medicine
- Allergology
- Respiratory Medicine
Background:
- Allergic asthma and rhinitis are significant occupational health concerns.
- Cellulase, an enzyme used in various industries, can be an allergen.
- Accurate diagnosis of enzyme-induced respiratory allergies is crucial for workplace safety.
Purpose of the Study:
- To develop and evaluate a challenge test for diagnosing cellulase-induced allergic asthma and rhinitis.
- To simulate occupational exposure conditions in a controlled laboratory setting.
Main Methods:
- Eleven individuals sensitized to cellulase underwent chamber challenge tests.
- Four enzyme-lactose mixtures with varying cellulase concentrations were used.
- Cellulase dust was generated using pressurized air, and concentrations were measured immunochemically.
Main Results:
- Respiratory symptoms (nasal, pharyngeal, bronchial) were elicited at cellulase concentrations from 1 to 1300 microg/m3.
- A clear dose-response relationship was observed for symptoms during repeated challenge tests.
- Skin symptoms were reproduced in two individuals.
Conclusions:
- The developed challenge test is a practical method for diagnosing cellulase allergy.
- The test effectively simulates workplace exposure to elicit specific respiratory symptoms.
- This diagnostic tool aids in identifying and managing occupational allergies.