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Related Experiment Videos

Use tests: ROAT (repeated open application test)/PUT (provocative use test): an overview.

T Nakada1, J J Hostynek, H I Maibach

  • 1Department of Dermatology, Showa University School of Medicine, Tokyo, Japan.

Contact Dermatitis
|July 21, 2000
PubMed
Summary

Use tests help determine allergen relevance after patch testing, but results vary. Further research is needed to standardize these tests for accurate clinical diagnosis.

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Area of Science:

  • Dermatology
  • Allergology
  • Clinical Immunology

Background:

  • Patch testing identifies allergens, but clinical relevance requires further assessment.
  • Use tests, including provocative use test (PUT) and repeated open application test (ROAT), evaluate allergen relevance.
  • Current use tests show variability, with negative results in up to 50% of cases despite reliable patch tests.

Purpose of the Study:

  • To define the clinical relevance of allergen exposure identified by patch testing.
  • To explore the limitations and potential improvements of use tests for diagnosing allergic contact dermatitis.
  • To highlight the need for standardized measurements and further controlled observations in use testing.

Main Methods:

  • Review and analysis of existing data on use tests (PUT and ROAT).

Related Experiment Videos

  • Observation of regional skin reactivity and percutaneous penetration differences.
  • Comparison of reaction differences between normal and diseased skin.

    • Main Results:

    • Use tests can be negative even with reliable patch tests, indicating the patient's threshold was not met.
    • Significant variations in skin reactivity and allergen penetration exist across different body sites.
    • Reactions may differ between normal and diseased skin, with potential for positive results on compromised skin.

    Conclusions:

    • Use testing holds potential for refining evidence-based diagnosis of clinical allergen relevance.
    • Standardization of measurements and further controlled studies are necessary for validation.
    • Understanding regional and skin condition-dependent differences is crucial for accurate interpretation of use test results.