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Cloning of cDNA encoding ethylene-responsive element binding protein-5 in the cultured cells of Nicotiana tabacum
Y Ashida1, N Yokobatake, C Kohchi
1Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|July 21, 2000
Abstract:
We have isolated a full-length cDNA clone from the cultured cells of Nicotiana tabacum, of which the deduced 225 amino acid sequence showed significant homology to the known ethylene-responsive element binding proteins (EREBPs) from tobacco and Arabidopsis. Although the clone shared substantial homology with an Arabidopsis ethylene-responsive element binding factor-3 (AtERF-3) throughout the full length, such a clone had not been found in N. tabacum cells yet; the clone was found to be 5th homologue of EREBPs on N. tabacum.