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Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA
H Inagaki1, T Hayashi, Y Matsushima
1Department of Chemistry, National Industrial Research Institute of Nagoya, Japan. hinagaki@nirin.go.jp
DNA Sequence : the Journal of DNA Sequencing and Mapping
|July 21, 2000
Abstract:
We have isolated rat mitochondrial transcription factor A (Tfam; formerly known as mtTFA) cDNA clones from a rat cerebellum cDNA library using human Tfam cDNA as a probe. The deduced amino acid sequence of r-Tfam shows 62% and 89% overall identity to human and mouse Tfam, respectively. We also show the presence of two r-Tfam isoforms in testis as for mouse. Our findings suggest that the mechanisms underlying transcription of mitochondrial genes are conserved among rat, mouse, and human.