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Related Experiment Videos

Transient translocation of the B cell receptor and Src homology 2 domain-containing inositol phosphatase to lipid

R J Petrie1, P P Schnetkamp, K D Patel

  • 1Departments of Biochemistry and Molecular Biology and Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada.

Journal of Immunology (Baltimore, Md. : 1950)
|July 21, 2000
PubMed
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B cell receptor (BCR) signaling involves lipid rafts, which sequester early events that regulate calcium release. Disrupting these membrane microdomains alters BCR redistribution and calcium flux during B cell activation.

Area of Science:

  • Cell Biology
  • Immunology
  • Biochemistry

Background:

  • Membrane microdomains, or lipid rafts, compartmentalize signaling molecules.
  • Lipid rafts play a role in receptor-mediated signal transduction.

Purpose of the Study:

  • To investigate the role of lipid rafts in B cell receptor (BCR) signaling.
  • To determine how lipid rafts affect BCR redistribution and downstream signaling events.

Main Methods:

  • Studied primary human B lymphocytes and Ramos B cells.
  • Utilized cholesterol sequestration to disrupt lipid rafts.
  • Assessed BCR redistribution, tyrosine kinase phosphorylation, and calcium release.

Main Results:

  • BCR stimulation caused redistribution of engaged BCRs to lipid rafts and tyrosine kinase phosphorylation.

Related Experiment Videos

  • Cholesterol sequestration prevented BCR redistribution but not tyrosine kinase activation.
  • Raft disruption enhanced intracellular calcium release, indicating raft sequestration of calcium flux regulators.
  • BCR stimulation led to SHIP translocation into lipid rafts.

    • Conclusions:

    • Lipid rafts are crucial for compartmentalizing early BCR signaling events.
    • Rafts may sequester signaling molecules that down-regulate calcium release.
    • SHIP translocation to rafts is a key event in BCR-mediated signaling regulation.