Related Experiment Video
Updated: Oct 15, 2026

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
Published on: September 17, 2016
A novel 'two-component' protein containing histidine kinase and response regulator domains required for sporulation
M Virginia1, C L Appleyard, W L McPheat
1Department of Biochemistry, University of Dundee, UK.
Abstract:
We have characterised a-novel Aspergillus nidulans gene encoding a 'two-component' signalling protein (tcsA). tcsA encodes both a histidine kinase domain and a response regulator domain similar to those found in bacterial, lower eukaryotic and plant members of the two-component family of proteins, while two PAS domains in the amino-terminal region of the predicted tcsA product may monitor the signal which regulates a tcsA histidine kinase-response regulator phosphorelay. While tcsA is nonessential for vegetative growth, cells lacking the gene are unable to produce conidia on standard Aspergillus growth media. However, tcsA is not absolutely required for production since this defect is suppressed by growth on 1 M sorbitol.
Related Concept Videos
Gene Regulation During Sporulation
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Endospores and Sporulation
Regulation of Bacterial Virulence
Yeast Signaling
Regulation of the Unfolded Protein Response
