Use of immunofluorescent localization in the normal guinea pig eye to differentiate three autoantisera

Three distinct immunofluorescent patterns were demonstrated in normal guinea pig eyes by autoantisera prepared to three different ocular tissue preparations. Anti-U serum (antiserum to ureal homogenate) exhibited specific fluorescence in the area of Bruch's membrane. Anti-P serum (antiserum to a suspension of the sedimentable portion of retina homogenate) exhibited specific fluorescence in the outer segments of the photoreceptor cells. Anti-S serum (antiserum to retinal homogenate extract) demonstrated specific fluorescence in the outer portion of the retina extending from the outer plexiform layer to the outer segments of the photoreceptor cells. The ability of anti-S and anti-P sera immunofluorescent activities to cross species lines was demonstrated.