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Published on: February 18, 2014
Analytical techniques for determining biotin
E Livaniou1, D Costopoulou, I Vassiliadou
1Institute of Radioisotopes/Radiodiagnostic Products, National Center for Scientific Research Demokritos, Athens, Greece.
Journal of Chromatography. A
|July 25, 2000
Summary
Biotin, a vital B-complex vitamin, is crucial for cell function and treating disorders like multiple carboxylase deficiency. This review evaluates analytical methods for measuring biotin in biological fluids, pharmaceuticals, and food for accurate diagnosis and monitoring.
Area of Science:
- Biochemistry
- Nutritional Science
- Analytical Chemistry
Background:
- Biotin (vitamin B7) is essential for cellular biochemical processes.
- Renewed interest stems from its role in treating inherited multiple carboxylase deficiency.
- Accurate biotin level determination is vital for clinical diagnosis and treatment monitoring.
Purpose of the Study:
- To review and evaluate analytical methods for biotin measurement.
- To cover methods applicable to biological fluids, pharmaceuticals, and food products.
Main Methods:
- Literature review of reported analytical methods for biotin determination.
- Evaluation of method characteristics and applicability to various sample types.
Main Results:
- Multiple analytical techniques exist for biotin quantification.
- Methods vary in sensitivity, specificity, and sample throughput.
- Suitability for clinical, pharmaceutical, and food analysis differs among methods.
Conclusions:
- Accurate biotin analysis is critical for managing deficiency disorders and ensuring product quality.
- Selection of appropriate analytical methods depends on the sample matrix and analytical goals.
- Further refinement of methods may enhance diagnostic and quality control capabilities.
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