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A new modular chemiluminescence immunoassay analyser evaluated
A Ognibene1, C J Drake, K Y Jeng
1Laboratory of Endocrinology and Toxicology, Azienda Ospedaliera Careggi, Firenze, Italy. ago@flownet.it
Clinical Chemistry and Laboratory Medicine
|July 25, 2000
Summary
The Abbott Architect 2000 analyzer demonstrates excellent performance for thyroid-stimulating hormone (TSH), free thyroxine (fT4), and testosterone assays. It offers superior reproducibility and functional sensitivity compared to other chemiluminescence immunoassay systems.
Area of Science:
- Clinical Chemistry
- Immunoassay Technology
- Analytical Performance Evaluation
Background:
- Chemiluminescence (CL) immunoassays are widely used in clinical diagnostics.
- Evaluating new automated immunoassay analyzers is crucial for improving diagnostic accuracy and efficiency.
- The Abbott Architect 2000 is a novel modular CL immunoassay analyzer.
Purpose of the Study:
- To assess the analytical performance of the Abbott Architect 2000 analyzer.
- To compare the Architect 2000 with existing CL immunoassay systems (DPC Immulite, Chiron Diagnostics ACS-180/Centaur).
- To evaluate TSH, fT4, and testosterone assays on the new platform.
Main Methods:
- Comparative analytical evaluation of the Abbott Architect 2000 against DPC Immulite and Chiron Diagnostics ACS systems.
- Assays performed included TSH, fT4, and testosterone.
- Reference methods used were equilibrium dialysis for fT4 and GC-MS for testosterone.
Main Results:
- The Abbott Architect 2000 exhibited superior functional sensitivity for TSH (0.0012 mU/L) and testosterone (0.38 nmol/L).
- Excellent correlation was observed between the Architect 2000 and ACS-180 for all tested analytes.
- The Architect 2000 demonstrated the best reproducibility (CV < 6%) for all three analytes in both within- and between-run precision studies.
Conclusions:
- The Abbott Architect 2000 analyzer provides high analytical performance for TSH, fT4, and testosterone assays.
- Its superior sensitivity and reproducibility make it a reliable option for clinical laboratories.
- The Architect 2000 shows promise as an advancement in automated chemiluminescence immunoassay technology.