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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Published on: October 16, 2018
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Novel mouse type D endogenous proviruses and ETn elements share long terminal repeat and internal sequences
1Terry Fox Laboratory, British Columbia Cancer Agency, and Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada. dixie@interchange.ubc.ca
Journal of Virology
|July 25, 2000
Summary
Early transposons (ETn) are mobile mutagens in mice. This study reveals they likely evolved from MusD retroviruses, explaining their mobility and retrotransposition mechanism.
Area of Science:
- Genetics
- Molecular Biology
- Genomics
Background:
- Early transposons (ETn) are active retrotransposons in the mouse genome.
- Their mobility mechanism was previously unknown due to lack of coding sequences.
- ETn elements possess long terminal repeats (LTRs) and other retroviral features.
Purpose of the Study:
- To elucidate the mobility mechanism of the ETn family of sequences.
- To identify the genetic elements responsible for ETn retrotransposition.
- To understand the evolutionary origin of the ETn family.
Main Methods:
- Bioinformatic searches for retroviral homology within ETn elements.
- Isolation and characterization of mouse endogenous proviral elements.
- Comparative analysis of MusD and ETn sequences across different mouse strains.
Main Results:
- A novel type D retroviral pol homology was identified in ETn elements.
- Two mouse endogenous proviral elements, termed MusD, were isolated, possessing gag, pro, and pol genes similar to simian type D viruses.
- MusD elements predate the mobile ETn subfamily, and their LTRs and regulatory regions are closely related to ETn elements.
- Evidence suggests ETn elements were formed by recombination, replacing MusD coding sequences with unrelated DNA.
Conclusions:
- The ETn family likely originated from MusD retroviruses through recombination events.
- ETn transcripts utilize proteins provided by MusD proviruses for retrotransposition.
- This study reveals the evolutionary origin and mobility mechanism of the ETn retrotransposon family.
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