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Related Experiment Videos

Gentamicin release from hydroxyapatite/poly(ethyl methacrylate)/poly(methyl methacrylate)composites.

R P del Real1, S Padilla, M Vallet-Regí

  • 1Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense, Madrid 28040, Spain.

Journal of Biomedical Materials Research
|July 25, 2000
PubMed
Summary

Gentamicin sulfate release from hydroxyapatite composites occurs in three stages over 70 days, with a significant portion released within the first 16 days. A carbonate hydroxyapatite layer formed on samples post-release.

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Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Materials Chemistry

Background:

  • Hydroxyapatite (HA) and methacrylate polymers are used in biomedical applications.
  • Gentamicin sulfate (GEN) is a common antibiotic for preventing implant-associated infections.
  • Controlling drug release from biomaterials is crucial for therapeutic efficacy.

Purpose of the Study:

  • To investigate the release kinetics of gentamicin sulfate (GEN) from hydroxyapatite (HA), poly(methyl methacrylate) (PMMA), and poly(ethyl methacrylate) (PEMA) composite samples.
  • To characterize the drug release profile over an extended period (70 days).
  • To analyze the surface changes of the composite materials after drug release.

Main Methods:

  • Drug release study conducted in simulated body fluid (SBF) at 37°C for 70 days.

Related Experiment Videos

  • Periodic replacement of the release medium.
  • Quantification of GEN concentration using the o-phthalaldehyde method.
  • Surface characterization of composite samples post-release.

    • Main Results:

    • The GEN release profile exhibited three distinct stages: a rapid release phase (<10 hours, ~30%), a slower phase (10 hours to 16 days, ~60%), and a very slow final phase (16 to 70 days, ~10%).
    • The release data correlated with the Cobby et al. model, fitting a third-order polynomial to the fraction of GEN released versus the square root of time.
    • Post-release analysis revealed the formation of a carbonate hydroxyapatite layer on the composite surfaces.

    Conclusions:

    • The HA/PMMA/PEMA composites demonstrate a multi-phasic release pattern for gentamicin sulfate.
    • The observed release kinetics are consistent with established mathematical models for drug diffusion and degradation.
    • The formation of a carbonate hydroxyapatite layer suggests potential bioactivity and influences the long-term behavior of the composite material.