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Lysozyme separation by hollow-fibre ultrafiltration.
1Department of Engineering Science, University of Oxford, Parks Road, OX1 3PJ, Oxford, UK
Biochemical Engineering Journal
|July 26, 2000
Summary
This study purified lysozyme from chicken egg white using hollow-fibre ultrafiltration. Optimized hydrodynamics and transmembrane pressure increased productivity without affecting lysozyme purity.
Area of Science:
- Biochemistry
- Protein Purification
- Membrane Technology
Background:
- Lysozyme, an enzyme found in egg white, has various industrial applications.
- Efficient purification methods are crucial for obtaining high-purity lysozyme.
- Hollow-fibre ultrafiltration offers a potential method for protein separation.
Purpose of the Study:
- To investigate the purification of lysozyme from chicken egg white using hollow-fibre ultrafiltration.
- To evaluate the impact of system hydrodynamics and transmembrane pressure on lysozyme purification efficiency.
- To determine optimal conditions for maximizing lysozyme purity and productivity.
Main Methods:
- Chicken egg white was processed using hollow-fibre ultrafiltration with a 30kDa molecular weight cut-off (MWCO) polysulphone membrane.
- System hydrodynamics were manipulated to assess their effect on permeate flux and lysozyme transmission.
- Transmembrane pressure (TMP) was varied to determine its influence on purification performance.
- Diafiltration mode was employed for extended purification operations.
Main Results:
- Lysozyme was preferentially transmitted through the membrane, while other egg white proteins were largely retained.
- Improved system hydrodynamics increased permeate flux and overall productivity without compromising lysozyme transmission.
- Permeate flux and productivity increased with TMP up to 0.7 bar, after which they plateaued.
- TMP did not significantly affect lysozyme transmission or purity.
- Diafiltration enabled the recovery of moderately pure lysozyme (80-90%) with extended operation.
Conclusions:
- Hollow-fibre ultrafiltration is an effective method for lysozyme purification from chicken egg white.
- System hydrodynamics and transmembrane pressure are key parameters for optimizing ultrafiltration performance.
- Optimized conditions can lead to enhanced productivity while maintaining lysozyme purity.
- Diafiltration offers a viable approach for achieving moderately pure lysozyme in longer processing runs.