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Published on: September 6, 2016

An antibody-lectin sandwich assay for asialo-LDL levels in atherosclerosis

A McDowell1, I S Young, G B Wisdom

  • 1Peptide and Protein Engineering Group, School of Biology and Biochemistry, The Queen's University, Belfast, UK-Northern Ireland.

Biochemical Society Transactions
|July 26, 2000
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.