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Related Experiment Videos

Nitrergic and peptidergic innervation in the developing rat heart.

T Shoba1, S S Tay

  • 1Department of Anatomy, Faculty of Medicine, National University of Singapore, Singapore.

Anatomy and Embryology
|July 26, 2000
PubMed
Summary

Autonomic efferent nerves develop early in the rat heart, preceding sensory and nitrergic (nitric oxide-producing) innervation. Neuropeptide Y and calcitonin gene-related peptide show distinct expression patterns during fetal development.

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Area of Science:

  • Developmental Neuroscience
  • Cardiovascular Physiology
  • Histochemistry and Immunohistochemistry

Background:

  • The developing heart is innervated by both autonomic and sensory nerves.
  • Nitric oxide and neuropeptides play crucial roles in cardiac function and development.
  • Understanding the precise timing and distribution of these innervations is vital.

Purpose of the Study:

  • To map the phenotypic expression and anatomical distribution of nitrergic and peptidergic innervation in the developing rat heart.
  • To determine the temporal sequence of autonomic efferent, sensory, and nitrergic innervation development.

Main Methods:

  • Reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry.
  • Immunohistochemistry using antibodies against neuronal nitric oxide synthase (nNOS), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP).

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  • Double-label immunohistochemistry for co-localization studies.

    • Main Results:

    • Neuropeptide Y (NPY)-immunoreactive fibers appeared earliest (16 days gestation), primarily innervating nodal/perinodal areas, followed by valves and ventricles.
    • Calcitonin gene-related peptide (CGRP)-positive fibers emerged later (19 days gestation), with extrinsic sensory origin, and showed dynamic density changes postnatally.
    • Nitric oxide synthase (NOS)-positive innervation paralleled CGRP-positive sensory innervation, with partial co-localization of NOS and CGRP in some fibers.

    Conclusions:

    • Neuropeptides and nitric oxide are expressed during the late gestational period in the rat heart.
    • Autonomic efferent innervation precedes sensory and nitrergic innervation in cardiac development.
    • Distinct temporal and spatial patterns characterize the development of different neuronal populations in the embryonic and neonatal heart.