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Related Experiment Videos

Neutrophil activation in immunoadsorption.

K Ota1, Y Shimizu, H Ichikawa

  • 1Department of Neurology, Neurological Institute, Kidney Center, Tokyo Women's Medical University, Japan.

Therapeutic Apheresis : Official Journal of the International Society for Apheresis and the Japanese Society for Apheresis
|July 26, 2000
PubMed
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Immunoadsorption therapy (IAT) alters white blood cell (leukocyte) activation markers. Neutrophil L-selectin decreased while Mac-1 increased, suggesting immune cell modulation during IAT for autoimmune disorders.

Area of Science:

  • Immunology
  • Cellular Biology
  • Medical Science

Background:

  • Immunoadsorption therapy (IAT) is a treatment for autoimmune diseases.
  • IAT is known to affect humoral immunity but its impact on cellular immunity, particularly leukocytes, is less understood.
  • Understanding leukocyte activation during IAT is crucial for assessing its overall immunomodulatory effects.

Purpose of the Study:

  • To investigate the effects of IAT on leukocyte activation in patients with neuroimmunological disorders.
  • To analyze changes in L-selectin (CD62L) and Mac-1 (CD11b) expression on neutrophils and mononuclear cells during IAT.

Main Methods:

  • Flow cytometry was used to continuously measure leukocyte L-selectin and Mac-1 expression in 6 patients undergoing IAT.
  • IAT was performed using specific plasma separation and adsorption devices.

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  • Leukocyte counts were also monitored throughout the therapy.

    • Main Results:

    • Neutrophil L-selectin expression decreased significantly during IAT, while mononuclear cell L-selectin showed a slight increase.
    • Neutrophil Mac-1 expression markedly increased by the end of IAT, with no significant change in mononuclear cells.
    • Leukocyte counts initially decreased then recovered, correlating with L-selectin and Mac-1 expression changes on neutrophils.

    Conclusions:

    • IAT induces specific changes in leukocyte activation markers, particularly neutrophils.
    • L-selectin downregulation and Mac-1 upregulation on neutrophils suggest their activation during IAT.
    • These findings indicate that IAT may modulate the circulating immune system through effects on leukocyte activation and counts.