Are the stainless steel electrodes inert?

The electrodes used for electroporation, electrofusion, and electrotransfection, as well as in electrochemotherapy should not interact with the object and release electrolysis products. We studied how far the stainless steel electrodes fulfil these requirements. The results showed that in the case of the exponential electric pulses with amplitude of 3 kV cm(-1) and time constant of 0.5 ms, the stainless steel electrodes released ferrous ions to a concentration of several micromoles per liter. We determined experimentally the dependence of the amount of the released ferrous ions on the pulse parameters and on the ionic strength of the medium. It was shown that the released ferrous ions should not be ignored. They could be of importance for the bleomycin electrochemotherapy.