Detergent solubilisation of baboon histocompatibility antigens and their use in prolonging liver allograft survival
Related Experiment Videos
Histocompatibility antigens have been solubilised from baboon spleen cell membranes by brief exposure to sodium deoxycholate (DOC). Preparations contained a high molecular weight species (DOCH-Ag) excluded by Sephadex G-200, and a low molecular weight species (DOCL-Ag) which eluted in the included fraction (molecular weight 46,000). The low molecular weight antigen was stable and was not sedimented by high speed centrifugation even after dialysis against a low molar salt solution. It elicited a delayed-type hypersensitivity response in sensitised baboons but not in normal animals. DOCL-Ag cultured in vitro with sensitised allogeneic lymphocytes produced a 1.3- to 24.9-fold stimulation, whereas when cultured with normal lymphocytes the maximum stimulation index was 2. Intravenous administration of donor DOCL-Ag (2 mg/kg) at the time of transplantation and without additional immunosuppression significantly prolonged the mean survival time of baboon liver allografts. The same treatment with donor DOCH-Ag did not prolong graft survival.
Histocompatibility antigens have been solubilised from baboon spleen cell membranes by brief exposure to sodium deoxycholate (DOC). Preparations contained a high molecular weight species (DOCH-Ag) excluded by Sephadex G-200, and a low molecular weight species (DOCL-Ag) which eluted in the included fraction (molecular weight 46,000). The low molecular weight antigen was stable and was not sedimented by high speed centrifugation even after dialysis against a low molar salt solution. It elicited a delayed-type hypersensitivity response in sensitised baboons but not in normal animals. DOCL-Ag cultured in vitro with sensitised allogeneic lymphocytes produced a 1.3- to 24.9-fold stimulation, whereas when cultured with normal lymphocytes the maximum stimulation index was 2. Intravenous administration of donor DOCL-Ag (2 mg/kg) at the time of transplantation and without additional immunosuppression significantly prolonged the mean survival time of baboon liver allografts. The same treatment with donor DOCH-Ag did not prolong graft survival.