Related Experiment Videos
Prolonged culture of human cryopreserved embryos with recombinant human leukemia inhibitory factor
Y Y Hsieh1, H D Tsai, C C Chang
1Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
Purpose:
To evaluate the efficiency of recombinant human leukemia inhibitory factor (LIF) in the prolonged culture of human cryopreserved-thawing embryos.
Methods:
After thawing, all embryos were divided into four groups: (1) Human tubal fluid (HTF), (2) HTF + LIF, (3) M3TH medium, and (4) M3TH medium plus LIF. Following prolonged culture, embryo development in each group was compared.
Results:
In embryo development from about the 2- to 4-cell to 9- to 16-cell stage, there were nonsignificant differences between each group. There was lower morula formation rate in group 1 (6.9%) than those in other groups (23.2%, 19.7%, 23.1%). The lower blastocyst formation in group 1 and 3 (0%, 0%) than those in group 2 and 4 (11.0%, 12.8%) were noted.
Conclusions:
LIF is beneficial for preimplantation embryos. LIF does not influence the early embryo development. LIF-supplemented HTF provided a similar culture environment for thawing embryos as LIF-supplemented M3TH medium.