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Updated: May 24, 2026

Rapid PCR Thermocycling using Microscale Thermal Convection
Published on: March 5, 2011
Gradient temperature hybridization using a thermocycler for RNase protection assays
1Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599-7295, USA. luzhang@med.unc.edu
Abstract:
The RNase protection assay (RPA) is a sensitive and quantitative assay for the detection of specific RNAs. Because some probes require an optimal temperature for efficient hybridization to the target RNAs, preliminary experiments to determine the optimal temperatures have to be done empirically, a process that is laborious and time-consuming. In order to simplify this process, a new gradient temperature hybridization procedure has been developed with the use of a thermocycler. This procedure eliminates the need for preliminary experiments to determine the optimal temperature of hybridization for a given probe.
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