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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
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Published on: March 22, 2016

Analysis of plus-strand primer selection, removal, and reutilization by retroviral reverse transcriptases

S J Schultz1, M Zhang, C D Kelleher

  • 1Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195-7242, USA.

The Journal of Biological Chemistry
|July 29, 2000
PubMed
Summary

Reverse transcriptase primer processing is crucial for viral DNA integration. Specific RNase H cleavage ensures correct primer removal and prevents repeated plus-strand DNA synthesis.

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