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Updated: Oct 15, 2026

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
Published on: May 26, 2011
Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving
P Obrdlik1, G Neuhaus, T Merkle
1Institut für Biologie II, Zellbiologie, Universität Freiburg, Schänzlestr. 1, D-79104, Freiburg, Germany.
Abstract:
Gbeta subunits from animals are anchored to membranes via Ggamma subunits. No Ggamma has been identified in plants to date. Using differential centrifugation of Arabidopsis and broccoli extracts, Gbeta was highly enriched in the microsomal pellet. Treatment of microsomes with detergents and salts indicates that plant Gbeta is located at the membrane surface and attached to membranes by hydrophobic interactions. Analysis of transgenic plants expressing Gbeta-GFP fusion proteins showed that mutations in the heptad repeat domain of Gbeta severely diminished their membrane association. We propose that plant Gbeta is anchored to membranes by an unknown protein similar to animal Gbeta by Ggamma, via coiled-coil formation.
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