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Updated: Jul 28, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
Synthesis of fluorescent probes directed to the active site gorge of acetylcholinesterase
J R Saltmarsh1, A E Boyd, O P Rodriguez
1Department of Chemistry, The University of Montana, Missoula 59812, USA.
Abstract:
Six organophosphorus compounds linked to fluorophore groups were prepared in an effort to selectively modify the active site of acetylcholinesterase and deliver probes to the gorge region. Two compounds that vary by the length of a methylene (CH2) group, pyrene-SO2NH(CH2)nNHC(O)CH2CH2P(O)(OEt)(F) (where n = 2 or 3) were found to be potent, irreversible inhibitors of recombinant mouse AChE (Ki approximately 10(5) M(-1) min(-1)). Size exclusion chromatography afforded a fluorescently-labeled cholinesterase conjugate.
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