Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modeling solid-to-solid biocatalysis: integration of six consecutive steps.

M J Michielsen1, C Frielink, R H Wijffels

  • 1Wageningen University, Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Biotechnion/Dreijencomplex, P.O. Box 8129/6700 EV Wageningen, The Netherlands.MARCO.MICHIELSEN@ALGEMEEN.PK.WAU.NL

Biotechnology and Bioengineering
|August 5, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of hydraulic retention time on community assembly and function of photogranules for wastewater treatment.

Water research·2020
Same author

Selective and energy efficient extraction of functional proteins from microalgae for food applications.

Bioresource technology·2018
Same author

Techno-Functional Properties of Crude Extracts from the Green Microalga Tetraselmis suecica.

Journal of agricultural and food chemistry·2018
Same author

Emotional face recognition in male adolescents with autism spectrum disorder or disruptive behavior disorder: an eye-tracking study.

European child & adolescent psychiatry·2018
Same author

Biorefinery of the macroalgae <i>Ulva lactuca</i>: extraction of proteins and carbohydrates by mild disintegration.

Journal of applied phycology·2018
Same author

Multi-Product Microalgae Biorefineries: From Concept Towards Reality.

Trends in biotechnology·2017
Same journal

Developing Anti-EGFR/Anti-HER2 Bifunctional Antibody for Solid Tumors by Protein Engineering.

Biotechnology and bioengineering·2026
Same journal

Bridging Organ-on-a-Chip and Omics: A Multi-Dimensional Frontier in Biomedical Research.

Biotechnology and bioengineering·2026
Same journal

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice.

Biotechnology and bioengineering·2026
Same journal

Characterization and Therapeutic Potential of a Novel Lytic Phage-Derived Endolysin PA16cLys Against Uropathogenic Pseudomonas aeruginosa Biofilms.

Biotechnology and bioengineering·2026
Same journal

Modeling Multiscale Architecture of Biofilm Extracellular Matrix and Its Role in Oxygen Transport.

Biotechnology and bioengineering·2026
Same journal

A Behavior-Integrated Potency Assay for Quantitative Evaluation of Extracellular Matrix Remodeling by Mesenchymal Stem/Stromal Cells.

Biotechnology and bioengineering·2026
See all related articles

A new model quantifies solid-to-solid salt conversion in bioreactors, integrating multiple steps like dissolution, bioconversion, and crystallization. This model accurately predicts component concentrations and solid phases during Ca-maleate to Ca-D-malate conversion.

Area of Science:

  • Biochemical Engineering
  • Chemical Engineering
  • Biocatalysis

Background:

  • Solid-substrate salt conversion is crucial in bioprocesses.
  • Accurate modeling is needed to optimize batch bioreactor performance.
  • Previous models lacked integration of multiple complex steps.

Purpose of the Study:

  • To develop a quantitative model for solid-salt to solid-product conversion in batch bioreactors.
  • To integrate six serial steps including dissolution, dissociation, bioconversion, complexation, and crystallization.
  • To validate the model using a specific bioconversion process.

Main Methods:

  • Developed a quantitative model integrating six serial steps: dissolution, dissociation, bioconversion, inactivation, complexation, and crystallization.

Related Experiment Videos

  • Assumed salt dissociation and ion complexation are at equilibrium.
  • Determined model parameters from independent experiments.

    • Main Results:

    • The model accurately predicted liquid-phase component concentrations (Ca-maleate, Ca(2+), maleate(2-), D-malate(2-), Ca-D-malate).
    • The model accurately predicted solid-phase amounts (Ca-maleate.H(2)O, Ca-D-malate.3H(2)O).
    • Model predictions were especially accurate with high initial solid phase amounts.

    Conclusions:

    • The developed quantitative model effectively describes solid-salt to solid-product conversion in batch bioreactors.
    • The model provides a valuable tool for optimizing bioprocesses involving salt conversions.
    • Accurate prediction of both liquid and solid phases is achievable with this integrated model.