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Analysis of urinary nucleosides. I. Optimisation of high performance liquid chromatography/electrospray mass
E Dudley1, S El-Sharkawi, D E Games
1Biochemistry Group, School of Biological Sciences, University of Wales, Singleton Park, Swansea SA2 8PP, UK.
Abstract:
In order to optimise the analysis of urinary nucleosides by high performance liquid chromatography/mass spectrometry (HPLC/MS), the HPLC separation of these compounds was performed at different 'flow rates' and 0.2mL/min was found to give both a better separation and ionisation. The ionisation conditions were optimised to give the best intensity of the molecules quasi-molecular ions. The ion distribution profile and ionisation in both positive and negative mode were examined and the detection of the protonated molecule in positive mode chosen for further analysis. The limits of detection of the method developed are reported and representative LC/MS and LC/MS/MS spectra shown. Typical urinary nucleoside chromatograms are presented.