Lymphocyte proliferation is possible with low concentrations of glycyl-glutamine

The positive effect of glutamine on lymphocyte proliferation has previously been described. Its dipeptide glycyl-glutamine (GlyGln) is more stable than pure glutamine in aqueous solutions. The aim of our study was to investigate the relationship between lymphocyte proliferation and varying concentrations of glycyl-glutamine in vitro. Isolated human lymphocytes were stimulated with the mitogens phytohaemagglutinin (PHA), Concanavalin A (ConA), pokeweed mitogen (PWM), and Staphylococcus aureus (SAC). Glycyl-glutamine was added to yield final concentrations of 0-2 mmol/l. Overall, minimal concentrations of 0.01 mmol/l glycyl-glutamine were sufficient to enhance lymphocyte proliferation over baseline (glutamine-free) levels. No difference was found between concentrations in the "physiological" range of 0.4 mmol/l and very low concentrations (0.04-0.1 mmol/l) with SAC, ConA and PWM. Increasing the concentration beyond 0.4 mmol/l (up to 2.0 mmol/l) offered further gain with PHA-stimulation only. Lymphocyte proliferation under in vitro polyclonal stimulation is maintained even at very low concentrations of glycyl-glutamine. Raising the concentration above the equivalent of physiological levels does not seem to provide further benefit.