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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
PCR use of highly conserved DNA regions for identification of Sinorhizobium meliloti
M Sánchez-Contreras1, J Lloret, M Martín
1Departamento de Biología, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
Abstract:
A PCR identification method in which four primers that recognize homologous conserved regions in the Sinorhizobium meliloti genome are used was developed and tested. The regions used for identification were the nodbox 4 locus, which is located in one of the symbiotic megaplasmids, and the mucR gene, which is located in the chromosome. The new method was used to establish a collection of S. meliloti strains from polluted soils.
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