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Related Experiment Videos

Why do we still find anonymous ESTs?

S Cirera1, A K Winterø, M Fredholm

  • 1The Royal Veterinary and Agricultural University, Department of Animal Science and Animal Health, Division of Genetics and Breeding, Grønnegårdsvej 3, 1870 Frederiksberg C, Denmark.

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|August 2, 2000
PubMed
Summary
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Investigating anonymous pig small intestine expressed sequence tags (ESTs) revealed that some novel transcripts may represent new genes or divergent sequences. Further analysis is needed to identify the origins of unrecognized ESTs.

Area of Science:

  • Genomics
  • Bioinformatics

Background:

  • Public sequence databases have rapidly expanded, yet many expressed sequence tags (ESTs) remain unidentified.
  • Anonymous ESTs pose challenges in understanding gene function and evolutionary relationships.

Purpose of the Study:

  • To investigate the reasons behind the lack of similarity for anonymous pig small intestine ESTs in public databases.
  • To explore hypotheses including novel genes, alternative splicing, sequence divergence, and data inaccuracies.

Main Methods:

  • Selection of 20 anonymous pig small intestine ESTs from a larger pool.
  • Total sequencing of EST inserts.
  • Zoo-blot and Northern-blot analyses were performed.

Main Results:

Related Experiment Videos

  • Out of 20 analyzed ESTs, 7 showed significant matches to existing database entries.
  • 13 ESTs continued to lack significant database hits after analysis.
  • Results provide insights into potential novel transcripts and sequence variations.

    • Conclusions:

    • The study highlights the presence of unidentified transcripts in pig small intestine EST data.
    • Findings support hypotheses such as novel gene discovery and significant sequence divergence.
    • Further research is necessary to fully characterize the anonymous ESTs.