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[Correlation analysis of radiation effects on potential doubling time (Tpot) for human nasopharyngeal carcinoma
1Department of Radiology, Changzheng Hospital, 2nd Military Medical University, Shanghai.
Objective:
To understand radiation-induced cell kinetic changes of human nasopharyngeal carcinoma CNE cells and clarify cell biology basis of repopulation.
Methods:
Either exponential growing stage or plateau stage human nasopharyngeal carcinoma CNE cells were measured by BrdUrd/DNA bivariate flow cytometry for phase fraction, BrdUrd labeling index, S phase duration, potential doubling time (Tpot), etc. Correlation analysis of Tpot with other kinetic parameters was made.
Results:
(1) For exponentially growing CNE cells, shortening of Tpot after irradiation was closely correlated with increased BrdUrd labeled S phase fraction (P < 0.05), whereas no correlation was found between Tpot and other kinetic parameters measured; (2) for plateau stage CNE cells, shortening of Tpot with various radiation doses was closely correlated with decreased G0/G1 phase fraction (P < 0.05) and increased G2M phase fraction (P = 0.002), whereas no correlation was found between Tpot and other parameters measured.
Conclusion:
In a range of 0-8 Gy of irradiation, an increased accelerated repopulation rate was resulted from increasing radiation dose. Recruitment of G0 cells into cell cycle might play a major role in radiation-induced accelerated repopulation for plateau stage CNE cells, while it was contributed by an increased DNA synthesis manifested by an increased BrdUrd labeled S phase fraction for exponential growing CNE cells.
