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Updated: Oct 15, 2026
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
Published on: June 12, 2018
[Detection of telomerase activity in bronchoscopic brush-off cells in lung cancer patients]
Objective:
To investigate the diagnostic significance of the detection of telomerase activity in the exfoliated cells obtained from fiberobronchoscopic brushing.
Methods:
The techniques of TRAP-PCR-ELISA and TRAP-silver staining were employed to detecte telomerase activity in 57 cases with pulmonary diseases.
Results:
Telomerase activity in the lesion side of lung cancer patients (N = 23) was significantly higher than that in the contralateral side of the same patient (P < 0.05), and in patients with pneumonia (P < 0.05). Positive rate of telomerase activity detection in lung cancer patients (91.3%) was significantely higher than that of cytological examination (52.3%, P < 0.05). In 83.3% of the cases examined, exfoliated cells showing cytological dysplasia were found to be telomerase activity positive.
Conclusion:
Detection of telomerase activity in the exfoliated cells obtained from fiberobronchoscopic brushing may be an effective and sensitive method in the diagnosis of pulmonary malignant diseases.
