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[Allelic loss on chromosome 3p21-26 in nasopharyngeal carcinoma]
Objective:
To determine the frequency and extent of loss of heterozygosity (LOH) on chromosome 3p in nasopharyngeal carcinoma (NPC).
Methods:
Sixteen loci on chromosome bands 3p21-26 in 24 tumors was studied by using microsatellite analysis.
Results:
LOH on 3p21-26 was found in 16 of 24 tumors (66.7%). The highest frequency of the allelic loss was found in two adjacent loci D3S1620(50%, 11/22) and D3S1560 (50%, 9/18). Eight cases showed LOH in one contiguous region and 5 cases in more than one region. Sample 1, 3, 4, 7, 8, 10, 16, 17, 18, 19, and 22 had a contiguous stretch of allelic loss between D3S1297 and D3S1597.
Conclusion:
The smallest common LOH/deletion region seems likely to lie between D3S1620(3p26.2-26.3) and D3S1560(3p25.3). The allelic loss map defined here will facilitate finer mapping of putative tumor suppressor gene loci and positional cloning of such genes, which may play a role in carcinogenesis of NPC.