Related Experiment Videos
[Deletion of MTS1/p16 gene in human esophageal carcinoma]
1Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
Objective:
To investigate the alteration of MTS1/p16 gene in human esophageal carcinoma.
Methods:
A total of 60 human esophageal tissue specimens, comprising 30 squamous-cell carcinomas and 30 tumor-adjacent tissue specimens, were examined for homozygous deletion of p16 gene by using Southern blot hybridization and PCR method.
Results:
The results showed that no deletions were detected in 30 tumor-adjacent tissue samples. However, of 30 esophageal carcinoma specimens, 7 were found negative for p16 gene in Southern blot assay, and the deletion of the p16 gene in 5 samples were confirmed by PCR with a 16.7% p16 gene deletion rate.
Conclusion:
These data suggest that MTS1/p16 gene alterations may play a role in the progression of human esophageal carcinoma.