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Updated: Aug 27, 2026
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
[The structure and mRNA expression of p16 gene in AML cells]
Objective:
To explore the role of p16 gene in the pathogenesis of AML.
Methods:
PCR-SSCP and Northern blot were used to analyse the structure and mRNA expression of p16 gene in 16 cases of AML.
Results:
No deletion or mutation of p16 gene were found in all of the 16 cases. The p16 mRNA expression in 12 AML at diagnosis or relapse was significantly lower than that in 4 long term remission cases and normal controls. p16 mRNA expression was significantly higher in a case of AML with p53 mutation.
Conclusion:
p16 gene may not play an important role in the pathogenesis of AML. The high p16 expression in the patient with p53 mutation might reflect a kind of inbalance of the feedback cycle.
