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Related Experiment Video

Updated: Jun 25, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

Violet red bile 2 agar for stressed coliforms.

P A Hartman, P S Hartman, W W Lanz

Applied Microbiology

|April 1, 1975

Summary

A new autoclave-sterilizable violet red bile (VRB-2) agar significantly improved microbial counts in various food and water samples. This innovation offers a more reliable method for microbiological testing in diverse applications.

Area of Science:

Microbiology
Food Science
Water Quality Testing

Background:

Traditional microbiological media require preparation before each use, posing challenges for routine testing.
Violet red bile (VRB) agar is a common selective medium for coliform bacteria detection.

Purpose of the Study:

To evaluate the performance of a novel autoclave-sterilizable VRB agar (VRB-2) compared to standard VRB agar.
To assess the recovery rates of microbial counts using VRB-2 agar across different sample types.

Main Methods:

Microbial counts were performed on VRB-2 agar and a control (freshly boiled VRB agar).
Diverse sample matrices including water, cottage cheese, frozen vegetables, and raw milk were analyzed.

Main Results:

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Last Updated: Jun 25, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

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Published on: November 8, 2016

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

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VRB-2 agar yielded significantly higher microbial counts than the control medium.
Percentage increases in counts ranged from 112% (raw milk) to 217% (water).
The enhanced recovery was consistent across all tested sample types.

Conclusions:

Autoclave-sterilizable VRB-2 agar offers superior performance for microbial enumeration.
The development principle of VRB-2 agar is adaptable to other selective plating media.
This advancement can improve the efficiency and reliability of microbiological testing.