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Updated: Aug 12, 2026

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
Published on: March 6, 2013
[Segmented condensation of peptides on a solid phase using subtilisin complexed with sodium dodecyl sulfate]
S V Kolobanova1, I Iu Filippova, E N Lysogorskaia
1Lomonosov State University, Chemical Department, Vorob'evy gory, Moscow, Russia.
Abstract:
The subtilisin-sodium dodecyl sulfate complex was shown to catalyze the coupling of peptide segments on a solid phase in organic medium. By a two-stage enzymic condensation of peptide fragments on aminosilochrom (A) containing Met-Ala-Gly as a spacer, Dnp(or Boc)-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Met-Ala-Gly-A and Z-Ala-Ala-Glu(OMe)-Ala-Ala-Leu-Met-Ala-Gly-A were obtained. It was shown that the condensation products can be split off from the support using the Met residue cleavage by BrCN.
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