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[Correlation of interleukin 1 beta-converting enzyme(ICE) gene expression with gut epithelial cell apoptosis in
Objective:
To investigate the correlation of gut epithelial cell apoptosis with ICE, IL1 beta gene expression in septic mice.
Methods:
Sepsis was induced in mice by cecal ligation and puncture(CLP). Sham-operation group underwent the same manipulation but without CLP. 1, 3, 6 hours after CLP, gut epithelial cells, were isolated. IL1 beta, ICE gene expression was detected quantitatively by RT-PCR. Epithelial cell apoptosis was assessed by flow cytometric method and biochemically by DNA electrophoresis.
Results:
The survival rate of CLP mice was 1/10 as compared to 10/10 of sham-operation mice. IL1 beta, ICE mRNA expression in CLP mice was significantly higher than that in the sham-operation group(P < 0.01); IL1 beta mRNA expression was parallel to ICE mRNA expression. The number of epithelial cell apoptosis was correlated excellently to the level of ICE, IL1 beta mRNA expression. Epithelial cell apoptosis could not be detected in sham-operation group at the indicated time points.
Conclusion:
ICE, IL1 beta gene overexpression may be involved in the vulnerability of epithelial cell apoptosis in septic mice.