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[Compartmental distribution of hepatitis C virus quasispecies in mononuclear cells and liver]
Objective:
To explore the role of the infection of monouclear cells by hepatitis C virus(HCV) in the chronicity of HCV infection.
Methods:
Direct sequencing of the amplified hypervariable region (HVR) gene of HCV was used to distinguish main HCV quasispecies infecting non-hepatocytes from those infecting hepatocytes and circulating virions. The main HCV quasispecies in peripheral blood mononuclear cells(PBMC), liver-associated mononuclear cells (LAMC), serum and liver samples from 6 patients with chronic hepatitis C and from 6 patients with HCV-related cirrhosis were analysed and compared.
Results:
The main HCV qusispecies in the livers were different from those in PBMC in 3 transplanted cirrhotic patients and 5 patients with chronic hepatitis C. Different substitutions existed between PBMC and LAMC in 3 of 6 patients analysed. Mutations were observed within the first 50 amino acids of E2 gene, which induced amino acid change in 6 of 9 patients analysed.
Conclusion:
In chronically infected patients, the detection of HCV RNA in mononuclear cells is not due to the adsorption of circulating virions but a significant infection. The compartmental distribution of HCV quasispecies in hepatocytes and mononuclear cells could be involved in the chronicity of HCV infection.