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[The studies on MTS1/p16 gene deletion during transformation of human bronchial epithelial cells]
1Department of Elidogy, Cancer Institute Chines Academy of Medical Sciences, Beijing.
Objective:
To detect the deletion of MTS1/p16 and the expression of P16 protein during the transformation of human bronchial epithelial cell line M.
Methods:
Dual-color fluo-rescence in situ hybridization (FISH), immunohistochemical staining and western blot analysis were carried out.
Results:
No deletion of MTS1/p16 gene was found in MP18 cells of the cell line M. But partial deletions occurred in the MP36 and MP120 cells of the cell line M. Immunohistochemical analysis and western blot test showed lower expression of P16 protein in the MP120 cells than in MP20 cells.
Conclusions:
MTS1/p16 gene deletion contributes to the malignant transformation of the human bronchial epithelial cell line M. Also FISH is a useful technique in detecting gene deletion.