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[Identification of interleukin-1 beta converting enzyme gene expression in human kidney]
Objective:
To confirm that Interleukin-1 beta-converting enzyme(ICE) is a first identified apoptosis related gene in mammals.
Methods:
The expressions of ICE mRNA in human kidney tissue, different segment of nephron and glomerular resident cells were measured by using the method of microdissection, reverse transcription-polymerase chain reaction(RT-PCR) and Northern hybridization.
Results:
Compared with peripheral blood mononuclear lymphocyte weak expressions of ICE mRNA were noted not only in glomeruli and tubule but also in glomerular mesangial cells, glomerular endothelial cells and tubular epithelial cells of the human kidney.
Conclusion:
There is a widely distributed expression of ICE mRNA in the human kidney, which may provide a theoretical basis for the study of ICE gene in apoptosis of kidney.
