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Tertiary structure in E. coli tRNA Arg and tRNA Val.

K L Wong, P H Bolton, D R Kearns

Biochimica Et Biophysica Acta

|April 2, 1975

Summary

Proton NMR reveals a specific base pair in Escherichia coli tRNA Arg and tRNA Val, involving 4-thiouridine (s-4U) at position 8 and adenine (A) at position 14. This interaction significantly influences tRNA folding and molecular structure.

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Area of Science:

Molecular Biology
Biochemistry
Structural Biology

Background:

Transfer RNAs (tRNAs) are crucial molecules for protein synthesis.
The tertiary structure of tRNAs is essential for their function.
Modified nucleosides play vital roles in tRNA structure and stability.

Purpose of the Study:

To investigate the tertiary structure of Escherichia coli tRNA Arg and tRNA(1) Val.
To identify specific base pairings contributing to tRNA folding.
To understand the impact of modified nucleosides, like 4-thiouridine (s-4U), on tRNA structure.

Main Methods:

300 MHz proton Nuclear Magnetic Resonance (NMR) spectroscopy was employed.
Analysis of spectral resonances to identify specific base pairs.
Comparison of spectra for tRNAs containing s-4U versus Uridine (U).

Main Results:

A tertiary structure base pair between 4-thiouridine (s-4U) at position 8 and adenine (A) at position 14 was identified in both tRNA Arg and tRNA(1) Val.
The s-4U(8)-A(14) base pair results in a characteristic low-field resonance (14.8 ppm) in NMR spectra.
Conversion of s-4U to U shifts this resonance to 14.3 ppm, confirming the role of s-4U.
The s-4U(8)-A(14) base pair significantly constrains tRNA folding in solution.

Conclusions:

The s-4U(8)-A(14) tertiary base pair is a key structural feature in Escherichia coli tRNA Arg and tRNA(1) Val.
This base pair plays a critical role in stabilizing tRNA tertiary structure and influencing its overall conformation.
NMR spectroscopy is a powerful tool for elucidating tRNA structure and the contribution of modified nucleosides.

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