Related Experiment Videos
Absence of delayed chromosomal instability in a normal human fibroblast cell line after 125I iododeoxyuridine
C S Griffin1, A Neshasateh-Riz, R J Mairs
1Radiation and Genome Stability Unit, MRC, Harwell, Oxon, UK. c.griffin@har.mrc.ac.uk
Purpose:
To seek the delayed appearance of chromosomal abnormalities in human fibroblasts exposed to the Auger electron emitter 125I.
Materials And Methods:
Normal untransformed human fibroblasts, HF19, were exposed to a concentration of [I125]IUdR, which allowed the survival of 37% of clonogens. Chromosomal analysis using both conventional Giemsa and fluorescence in situ hybridization (FISH) was undertaken on non-clonal bulk cultures from 2 to 39 days after treatment.
Results:
The data show a declining level of unstable aberrations in the progeny of HF19 fibroblasts exposed to [I125]IUdR, eventually reaching control levels.
Conclusions:
The results provide evidence that [125I]IUdR does not induce ongoing chromosomal instability in long-term culture, and gives further support to the use of Auger-electron emitting radionuclides in the treatment and diagnosis of tumours.