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A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
Published on: July 6, 2009
Basic aspects of frozen storage of semen
1Institute of Zoology, Zoological Society of London, Regent's Park, NW1 4RY, London, UK. bill.holt@ioz.ac.uk
Animal Reproduction Science
|August 5, 2000
Abstract:
Basic concepts of cryopreservation and the causes of cryoinjury are reviewed. The possible roles of cryoprotectants and additives are considered in the context of their putative interactions with the sperm plasma membrane. Modern approaches to the laboratory assessment of spermatozoa after freeze-thawing are also briefly discussed.
