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IgM alloantibody detection by M-MPHA, a new solid-phase assay system
T Toyohiro1, I Yasuyuki, M Toshio
1Diagnostic Systems Division, Olympus Japan Co. Ltd., Ryumei-kan Building 4, Kanda-Surugadai 3, Chiyoda-ku, 101-0062, Tokyo, Japan. t-tamai@sales.olympus.co.jp
Abstract:
New IgM alloantibody detection system that uses anti-human IgM-antibody-sensitized magnetic particles mixed passive hemagglutination (IgM-M-MPHA) has been developed. IgM antibodies are well detected by direct agglutination using the saline tube test. Anti-IgM using IgM antibody detection is not used routinely. In our evaluation, IgM-M-MPHA showed good detection of IgM alloantibodies. When IgM-M-MPHA is used together with IgG-M-MPHA (anti-human IgG-antibody-sensitized magnetic particles mixed passive hemagglutination), alloantibodies can be distinguished easily whether it is IgG, IgM or both.